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CL-0774
$ 500.00
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1×10^6Cells/Vial×2Vials
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Product Introduction
General information
| Cell Name | WSU-DLCL2 |
| Synonyms | WSU-DLCL-2;WSU-DLCL(2);WSUDLCL2 |
| Cellosaurus Accession | CVCL_1902 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Round and oval cells, growing singly or in small suspension clusters |
| Disease | lymphoma cells |
| Tissue | Pleural effusion |
| Age | 41Y |
| Sex | Male |
Handling information
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:3-1:5 |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
Reference materials
| Background | WSU-DLCL2 was established in 1990 from pleural effusion collected from a 41-year-old male patient with relapsed/progressive B-cell non-Hodgkin lymphoma (B-NHL). The lymphoma was classified as intermediate-grade non-cleaved diffuse large cell lymphoma, transformed from low-grade follicular small cleaved cell lymphoma. WSU-DLCL2 harbors the EZH2 Y641F mutation, and corresponding exome and RNA sequencing datasets are publicly available for this cell line. |
| Biosafety Level | BSL-1 |
Documents
MSDSPublications
Fat Mass and Obesity-Associated Protein Contributes to Tumorigenesis and Drug Resistance of Diffuse Large B-Cell Lymphoma by Suppressing N6-Methyladenosine Methylation of Myc
Journal: KAOHSIUNG JOURNAL OF MEDICAL SCIENCES (2026) IF: 3.1
DOI: 10.1002/kjm2.70158
Product Cited: U-2932 Cells Line, HMy2.CIR Cells Line, Penicillin-Streptomycin Solution, 100 ×, WSU-DLCL2 Cells Line, SU-DHL-8 Cells Line
Inhibition of CDGSH iron?sulfur domain 2 exhibits tumor-suppressing effects on diffuse large B-cell lymphoma (DLBCL) by inducing ferroptosis through the regulation of the NRF2/SLC7A11/GPX4 pathway
Journal: TOXICOLOGY AND APPLIED PHARMACOLOGY (2024) IF: 3.3
DOI: 10.1016/j.taap.2024.117148
Product Cited: U-2932 Cells Line, WSU-DLCL2 Cells Line, HMy2.CIR] Cells Line, SU-DHL-4 Cells Line, OCI-Ly3 Cells Line
