PB180325
$ 40.00
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Product Introduction
Background
HEPES is all known as N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid, is a commonly used biological buffer. pKa (25℃) is 7.5 and an effective buffer range of pH is 6.8-8.2. The greatest advantage of HEPES is that to maintain the pH range of a solution for a longer period of time in an open environment.
HEPES cannot pass through biofilms. It is a chemical and enzymatic stable substance with limited effect on biochemical reactions, and the absorption of visible and ultraviolet light is extremely low. Therefore, it is generally considered that HEPES is an excellent biological buffer, and there is no toxic effect to the cells. The working concentration is 10-50 mM and the final concentration for cell culture is 10 mM.
Ingredient Statement
With
• 238.3 g/L HEPES
• Purified Water Dissolvent
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the pollution.
4. This product is 50 × concentrated solution and dilute it to to the desired concentration before use.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's 1 M HEPES Solution complies with ISO 13485, ISO 9001.
Specifications
| Concentration | 1 M |
| Volume | 100mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months |
| Shipping Condition | Ice bag |
Publications
Journal: Biomolecules (2023) IF: 5.500
DOI: 10.3390/biom13040587
Product Cited: RPMI-1640, Penicillin-Streptomycin Solution, 100×, 1 M HEPES Solution
Journal: COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS (2023) IF: 5.2
DOI: 10.1016/j.colsurfa.2023.131904
Product Cited: 1 M HEPES Solution
Journal: CELLULAR SIGNALLING (2023) IF: 4.8
DOI: 10.1016/j.cellsig.2023.110840
Product Cited: 1 M HEPES Solution
Journal: ENVIRONMENTAL TOXICOLOGY (2023) IF: 4.5
DOI: 10.1002/tox.23874
Product Cited: 1 M HEPES Solution
Journal: Brazilian Journal of Otorhinolaryngology (2023) IF: 2.200
DOI: 10.1016/j.bjorl.2022.06.005
Product Cited: TU177 Cells Line, FaDu Cells Line, LSC-1 Cells Line, FD-LSC-1 Cells Line, 1 M HEPES Solution
