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CL-0041
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Product Introduction
| Cell Name | BT-549 |
| Synonyms | BT 549;BT.549;BT549 |
| Cellosaurus Accession | CVCL_1092 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Disease | breast cancer cells |
| Tissue | Breast; Mammary gland |
| Age | 72Y |
| Sex | Female |
| Instructions | 1. Check all containers for leakage or breakage.<br/> 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+10 μg/mL Insulin [PB180432]+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:4 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 2-3 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | Freezing Medium (Serum-free & animal origin-free) [PB180438] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
| Background | The BT-549 cells was a papillary invasive ductal carcinoma with metastases present in 3 out of 7 regional lymph nodes. BT-549 cells established from this tissue are pleomorphic, containing epithelial-like components and multinucleated giant cells. |
| Biosafety Level | BSL-1 |
Documents
MSDSPublications
Journal: NUCLEIC ACIDS RESEARCH (2026) IF: 13.1
DOI: 10.1093/nar/gkag362
Product Cited: Hs 578T Cells Line, HCC1806 Cells Line, MDA-MB-436 Cells Line, BT-549 Cells Line, SK-BR-3 Cells Line, MCF 10A Cells Line, MDA-MB-361 Cells Line, MDA-MB-231 Cells Line
Journal: Small (2026) IF: 12.1
Product Cited: BT-549 Cells Line, MCF 10A Cells Line
Journal: CANCER LETTERS (2026) IF: 10.1
DOI: 10.1016/j.canlet.2026.218278
Product Cited: DMEM (High glucose) Medium, Penicillin-Streptomycin Solution, 100 ×, RPMI-1640 Medium, Fetal Bovine Serum, MDA-MB-468 Cells Line, MDA-MB-231 Cells Line, HCC1806 Cells Line, MCF 10A Cells Line, BT-549 Cells Line
Journal: Cell Death & Disease (2026) IF: 9.6
DOI: 10.1038/s41419-026-08482-x
Product Cited: MCF 10A Cell Complete Medium, Hs 578T Cells Line, HCC1937 Cells Line, MCF 10A Cells Line, BT-549 Cells Line, MDA-MB-231 Cells Line
Journal: FREE RADICAL BIOLOGY AND MEDICINE (2026) IF: 8.2
DOI: 10.1016/j.freeradbiomed.2025.12.060
Product Cited: BT-549 Cells Line, MDA-MB-231 Cells Line
