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Product Introduction
Background
DMEM (Dulbecco's Modified Eagle Medium) was developed on the basis of MEM medium. Compared with MEM medium, the content of amino acid increased by 2 times, the content of vitamin increased by 4 times, and the content of non-essential amino acid, trace iron ion and sodium pyruvate were increased by 4 times.
The glucose content of DMEM medium was originally designed as 1000 mg/L (low Glucose type), and then developed into 4500 mg/L (high Glucose type), which has been widely used in cell culture.
DMEM (High glucose) was widely used in fast growth, low adhesion cells, hybridoma myeloma cells, clone cells, DNA transfected transformation cells, various primary virus host cells, single cell culture and vaccine production.
DMEM (High glucose) contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors, so the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 4500 mg/L D-Glucose
• 4 mM L-Glutamine
• 3700 mg/L NaHCO₃
• 15 mg/L Phenol red
• 1 mM Sodium pyruvate
Without
• HEPES
Matters Need Attention
1. This product is only used for scientific research or further research, not for diagnosis and treatment.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's DMEM (High glucose) also meets the medical device registration certificate requirements and complies with ISO 13485, ISO 9001.
Specifications
| Concentration | 1× |
| Volume | 500mL 1000mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 1 EU/mL |
| pH | 7.0-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Related Products
Documents
Publications
Journal: EXPERIMENTAL CELL RESEARCH (2022) IF: 4.2
DOI: 10.1016/j.yexcr.2022.113455
Product Cited: McCoy's 5A, DMEM(High glucose)
Journal: MOLECULAR IMMUNOLOGY (2022) IF: 4.2
DOI: 10.1016/j.molimm.2022.11.019
Product Cited: DMEM(High glucose), Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×
Journal: ENVIRONMENTAL TOXICOLOGY (2022) IF: 4.109
DOI: 10.1002/tox.23618
Product Cited: Fetal Bovine Serum, DMEM (High glucose) Medium
Journal: European Review for Medical and Pharmacological Sciences (2022) IF: 3.8
DOI: 10.26355/eurrev_202211_30130
Product Cited: DMEM(High glucose), Penicillin-Streptomycin Solution, 100×
Journal: European Review for Medical and Pharmacological Sciences (2022) IF: 3.8
DOI: 10.26355/eurrev_202211_30370
Product Cited: DMEM(High glucose), Penicillin-Streptomycin Solution, 100×, Phosphate Buffer (PBS), 0.25% Trypsin Solution, 10 mg/mL Recombinant Human Insulin Solution
FAQs
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Q:1 Can basic culture medium be used if it is accidentally frozen at -20°C?
AnswerIf the cell culture medium is accidentally frozen, allow it to thaw completely at room temperature and gently mix before use. After thawing, check whether any precipitation has formed. If no precipitate is observed, the medium can be used normally. If precipitation is present, it indicates that certain components have precipitated out of solution; in this case, to avoid affecting cell growth, the medium is not recommended for further use.
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Q:2 What is the difference between DMEM high- glucose and low-glucose formulations, and how should one choose?
AnswerThe primary difference between high-glucose and low-glucose DMEM formulations is the glucose concentration. The choice should be based on the metabolic requirements of the cell line and the experimental objectives. High-glucose DMEM contains 4.5 g/L (25 mM) glucose, providing an abundant carbon source. It is suitable for metabolically active, rapidly proliferating cell lines, including many tumor cells, and supports high-density and long-term culture without rapid nutrient depletion. Low-glucose DMEM contains 1.0 g/L (5.6 mM) glucose, which is closer to physiological blood glucose levels. It is more appropriate for cells with lower metabolic rates, certain primary cell cultures, or experimental conditions that require physiological relevance, such as modeling normoglycemic conditions or studying glucose restriction. It is also suitable for cells sensitive to high glucose or for low-glucose stress studies.
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Q:3 Can I culture all my cells in DMEM medium?
AnswerIt is not recommended to culture all cell types in DMEM, as different cell lines have specific nutritional requirements and growth characteristics. Using an inappropriate medium may result in poor cell health, reduced proliferation, or even cell death. Although DMEM is widely used, its high-glucose formulation (4.5 g/L) and elevated amino acid content are not suitable for all cell types. For example, suspension lymphocyte cell lines such as Raji and Jurkat are better maintained in RPMI 1640 medium, whereas insect cell lines such as Sf9 require entirely different media, such as Grace’s or TNM-FH. In addition, primary cells and stem cells often require specific growth factors, hormones, or specialized commercial media to maintain their undifferentiated state and normal physiological functions. Therefore, when selecting a culture medium, it is essential to consult the official guidelines provided by authoritative cell repositories such as ATCC and DSMZ, and to strictly follow the recommended basal medium, serum concentration, and supplementation conditions optimized for the specific cell line.
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Q:4 If the basic culture medium is frozen at -20℃, will it affect its use?
AnswerIf the cell culture medium is accidentally frozen, observe whether there is precipitation after the medium is naturally thawed. If no precipitation is produced, the medium can be used normally. If precipitation occurs, it is recommended to discard it.
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Q:5 How long can I keep my culture media after supplementing with serum?
AnswerGenerally speaking, media can be used for up to 3-6 months after supplementation with serum， at 2 to 8℃ low temperature environment preservation.
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Q:6 My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
AnswerWe routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
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Q:7 How light sensitive is media? Should I also be protecting it from LED light?
AnswerWhile we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.When placed in a light-proof 4°C refrigerator with the door closed, the culture medium will be kept away from light.
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Q:8 I see a precipitate in my medium, and a change in the pH. What should I do?
AnswerThis is most likely a sign of bacterial or fungal contamination. We would recommend discarding the media, and trying to decontaminate the culture.
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Q:9 Where can I find the osmolality for Medium?
AnswerThe osmolality is listed in the COA for the particular lot number of the medium.
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Q:10 Do you provide customized culture media services?
AnswerYes. We can customize culture media with reduced components or changes in the concentration of existing components.
