DMEM/F12

Cat. No.: PM150312 Publications(350) Manual
DMEM/F12 - 1
  • DMEM/F12DMEM/F12 - 1

PM150312

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Product Introduction

Background

The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Ingredient Statement
With

• 3151 mg/L D-Glucose

• 2.5 mM L-Glutamine

• 2438 mg/L NaHCO₃

• 8.1 mg/L Phenol red

• 0.5 mM Sodium pyruvate

Without

• HEPES

Matters Need Attention

1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.

System Certification

GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's DMEM/F12 also meets the medical device registration certificate requirements and complies with ISO 13485, ISO 9001.

Specifications
Concentration
Volume 500mL 1000mL
Form Liquid
Bacteria Detection Negative
Fungi Detection Negative
Mycoplasma Detection Negative
Endotoxin Content < 3 EU/mL
pH 7.2-7.4
Animal Origin Ingredient Without
Green Features Sustainable packaging
Valid Period 24 months
Storage Condition This product can be stored at 2-8℃ for 24 months with shading light.
Shipping Condition Room Temperature

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Publications

FEZF2 inhibits the growth of triple-negative breast cancer cells through EZH2/PD-L1 and enhances anti-tumor immunity in vivo

Journal: Molecular & Cellular Toxicology (2023) IF: 1.7

DOI: 10.1007/s13273-023-00368-9

Product Cited: MCF 10A Cells Line, DMEM/F12, Donor Equine Serum, Penicillin-Streptomycin Solution, 100×, MDA-MB-231 Cells Line, MDA-MB-468 Cells Line, DMEM(High glucose), BT-549 Cells Line, RPMI-1640, MDA-MB-436 Cells Line

HIF-1α is positively associated with endometrial receptivity by regulating PKM2

Journal: JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH (2023) IF: 1.6

DOI: 10.1111/jog.15752

Product Cited: DMEM/F12 Medium

Expression of LINC00461 in breast cancer cells and its modulatory effect on miR-607/SLCLA3 axis

Journal: TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH (2023) IF: 0.6

DOI: 10.4314/tjpr.v22i4.2

Product Cited: MCF 10A Cells Line, MDA-MB-468 Cells Line, T-47D Cells Line, MCF7 [MCF-7] Cells Line, MDA-MB-231 Cells Line, DMEM/F12, Fetal Bovine Serum, Penicillin-Streptomycin Solution, 100×

Thorium inhibits human respiratory chain complex IV (cytochrome c oxidase)

Journal: Journal of Hazardous Materials (2022) IF: 14.2

DOI: 10.1016/j.jhazmat.2021.127546

Product Cited: MG-63 Cells Line, Hep G2 Cells Line, MEM(with NEAA), DMEM/F12, Fetal Bovine Serum

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