PM150310
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Product Introduction
Background
The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 3151 mg/L D-Glucose
• 15 mM HEPES
• 2.5 mM L-Glutamine
• 1200 mg/L NaHCO₃
• 8.1 mg/L Phenol red
• 0.5 mM Sodium pyruvate
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's DMEM/F12, with HEPES also meets the medical device registration certificate requirements and complies with ISO 13485, ISO 9001.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Publications
Journal: DRUG RESISTANCE UPDATES (2024) IF: 24.3
DOI: 10.1016/j.drup.2024.101080
Product Cited: DMEM/F12, with HEPES
Journal: Cell Stem Cell (2024) IF: 19.8
DOI: 10.1016/j.stem.2024.07.003
Product Cited: DMEM/F12 (Glucose free), with HEPES Medium, DMEM/F12, without pyruvate Medium, DMEM/F12, with HEPES Medium
Journal: Biology Open (2024) IF: 1.8
DOI: 10.1242/bio.061790
Product Cited: DMEM/F12, with HEPES Medium, Fetal Bovine Serum
Journal: International Heart Journal (2024) IF: 1.2
DOI: 10.1536/ihj.24-162
Product Cited: AC16 Cells Line, DMEM/F12, with HEPES Medium, 293T [HEK-293T] Cells Line
Journal: Biomaterials Science (2023) IF: 6.600
DOI: 10.1039/D2BM01968J
Product Cited: DMEM/F12, with HEPES Medium
