HCC1954

Name: HCC1954
Brand: Procell
SKU: CL-0756
Price: 500.0 USD
Availability: InStock

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Cat. No.: CL-0756

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CL-0756

$ 500.00

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1×10^6Cells/Vial×2Vials

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Product Introduction Documents Publications

Product Introduction

General information

Cell Name	HCC1954
Synonyms	HCC-1954;Hamon Cancer Center 1954
Cellosaurus Accession	CVCL_1259
Organism	Human
Growth Properties	Adherent
Morphology	Epithelial-like; vacuolated
Disease	breast cancer cells
Tissue	Breast; Duct; Mammary gland
Age	61Y
Sex	Female

Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.
Complete Medium	RPMI-1640 [PM150110]+10% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	1:4-1:8
Medium Renewal	2 to 3 times per week
Dissociation Duration	3-5 min
Subculturing Procedure	1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	The HCC1954 cell line is an epithelial cell line isolated in 1995 from a primary stage IIA, grade 3 invasive ductal carcinoma with no lymph node metastases from a 61-year-old Asian female. HCC1954 is positive for the epithelial cell-specific marker epithelial glycoprotein 2 and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR). HER2/neu is overexpressed as determined by ELISA.
Receptor Expression	Estrogen receptor, not expressed; progesterone receptor, not expressed
Gene Expression	epithelial glycoprotein 2 (EGP2); cytokeratin 19
Biosafety Level	BSL-1