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CL-0110
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Product Introduction
| Cell Name | HL-60 |
| Synonyms | HL 60;HL60 |
| Cellosaurus Accession | CVCL_0002 |
| Organism | Human |
| Growth Properties | Suspension |
| Morphology | Lymphoblast-like |
| Disease | leukemia cells |
| Tissue | Peripheral blood |
| Age | 36Y |
| Sex | Female |
| Instructions | 1. Check all containers for leakage or breakage.<br/> 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation.<br/> 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly. |
| Complete Medium | IMDM [PM150510]+20% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 3×10⁵-1×10⁶ cells/mL |
| Medium Renewal | 2 to 3 times per week |
| Subculturing Procedure | Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
| Background | HL-60 cells are a promyelocytic cell line. They were established from a 36-year-old Caucasian female diagnosed with acute myelomonocytic leukemia. HL-60 cells undergo spontaneous differentiation, a process that can be enhanced by butyrate, hypoxanthine, phorbol ester (PMA), DMSO (1%–1.5%), actinomycin D, and retinoic acid. HL-60 cells exhibit phagocytic activity and respond to chemotactic stimuli. The cells are positive for the myc oncogene. |
| Tumorigenic | Yes, in nude mice (subcutaneous myeloid tumors). Yes, in semi-solid media. |
| Receptor Expression | complement, expressed; Fc, expressed |
| Gene Expression | tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid, myc+ |
| Biosafety Level | BSL-1 |
Documents
MSDSPublications
Journal: CELL (2026) IF: 42.5
DOI: 10.1016/j.cell.2025.12.050
Product Cited: HL-60 Cells Line, NCI-H226 Cells Line, B95-8 Cells Line, NB4 Cells Line, HMY-1 Cells Line, PC-9 Cells Line
Journal: IMMUNITY (2026) IF: 26.3
DOI: 10.1016/j.immuni.2026.03.026
Product Cited: HL-60 Cells Line
Journal: Nature Communications (2026) IF: 15.7
DOI: 10.1038/s41467-026-69250-1
Product Cited: HL-60 Cells Line, Penicillin-Streptomycin Solution, 100 ×, SVEC4-10 Cells Line, 0.5% EDTA Solution, AML12 Cells Line, MC38 Cells Line, PUMC-HUVEC-T1 Cells Line, RPMI-1640 Medium, DMEM (High glucose) Medium, Fetal Bovine Serum, Phosphate Buffer (PBS, 1 ×), CT26.WT Cells Line
Journal: Advanced Science (2026) IF: 14.1
Product Cited: IMDM Medium, DMEM (High glucose) Medium, HL-60 Cells Line, RAW 264.7 Cells Line, H22 Cells Line, LX-2 Cells Line, HC11 Cells Line, RPMI-1640 Medium
Journal: Interdisciplinary Medicine (2026) IF: 13.6
DOI: 10.1002/inmd.70115
Product Cited: HL-60 Cells Line
