HL-60

Name: HL-60
Brand: Procell
SKU: CL-0110
Price: 420.0 USD
Availability: InStock

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Cat. No.: CL-0110

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Handling information

Instructions	1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line is a suspension cell line. Harvest the cell suspension by centrifugation; do not discard the culture medium directly.
Complete Medium	IMDM [PM150510]+20% Nutrient+1% Supplement
Incubation Atmosphere	Air, 95%; CO₂, 5%
Temperature	37℃
Subcultivation Ratio	3×10⁵-1×10⁶ cells/mL
Medium Renewal	2 to 3 times per week
Subculturing Procedure	Cultures can be maintained by either supplementing with fresh medium or exchanging medium via centrifugation. The recommended centrifugation conditions are 1200 rpm (approximately 250 × g) for 3 minutes.
Freeze Medium	General Freezing Medium [PB180436]
Storage Conditions	For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only.

Reference materials

Background	HL-60 cells are a promyelocytic cell line. They were established from a 36-year-old Caucasian female diagnosed with acute myelomonocytic leukemia. HL-60 cells undergo spontaneous differentiation, a process that can be enhanced by butyrate, hypoxanthine, phorbol ester (PMA), DMSO (1%–1.5%), actinomycin D, and retinoic acid. HL-60 cells exhibit phagocytic activity and respond to chemotactic stimuli. The cells are positive for the myc oncogene.
Tumorigenic	Yes, in nude mice (subcutaneous myeloid tumors). Yes, in semi-solid media.
Receptor Expression	complement, expressed; Fc, expressed
Gene Expression	tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid, myc+
Biosafety Level	BSL-1