MEM (Glucose free), with L-alanyl-L-glutamine, HEPES
Manual
PM150460
$ 30.00
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Product Introduction
Background
MEM medium (Minimum Essential Medium) was developed on the basis of Eagle basic medium. It is one of the most basic and widely used culture medium, and one of the most commonly used culture medium in animal cell culture. MEM medium contains 12 kinds of essential amino acids, glutamine and 8 vitamins, which is simple, mainly used in the culture of adherent cells.
MEM, with NEAA medium is added L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and glycine on the basis of MEM medium. These 7 kinds of NEAA, can reduce the side effects of producing non-essential amino acids during cell culture and promote cell proliferation and metabolism.
MEM medium without glucose can be added to the concentration of glucose at will according to the research needs, which is convenient and quick.
Ingredient Statement
With
• 25 mM HEPES
• 2 mM L-Alanyl-L-Glutamine
• 2200 mg/L NaHCO₃
• 10 mg/L Phenol red
• Earle's Balanced salt
Without
• D-Glucose
• NEAA
Matters Need Attention
1. This product is only used for scientific research or further research, not for diagnosis and treatment.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's MEM (Glucose free), with L-alanyl-L-glutamine, HEPES complies with ISO 13485, ISO 9001.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Related Products
Documents
FAQs
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Q:1 I purchased MEM without NEAA, Should I add NEAA separately, and what is the recomended concentration?
AnswerYes, non-essential amino acids (NEAA) should be supplemented when using MEM without NEAA. We recommend using our MEM Non-Essential Amino Acids Solution (NEAA), catalog number PB180424. For preparation, add 1 mL of the NEAA solution to 99 mL of MEM medium, corresponding to a final concentration of 1% (v/v).
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Q:2 If the basic culture medium is frozen at -20℃, will it affect its use?
AnswerIf the cell culture medium is accidentally frozen, observe whether there is precipitation after the medium is naturally thawed. If no precipitation is produced, the medium can be used normally. If precipitation occurs, it is recommended to discard it.
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Q:3 How long can I keep my culture media after supplementing with serum?
AnswerGenerally speaking, media can be used for up to 3-6 months after supplementation with serum， at 2 to 8℃ low temperature environment preservation.
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Q:4 My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
AnswerWe routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
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Q:5 How light sensitive is media? Should I also be protecting it from LED light?
AnswerWhile we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.When placed in a light-proof 4°C refrigerator with the door closed, the culture medium will be kept away from light.
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Q:6 I see a precipitate in my medium, and a change in the pH. What should I do?
AnswerThis is most likely a sign of bacterial or fungal contamination. We would recommend discarding the media, and trying to decontaminate the culture.
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Q:7 Where can I find the osmolality for Medium?
AnswerThe osmolality is listed in the COA for the particular lot number of the medium.
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Q:8 Do you provide customized culture media services?
AnswerYes. We can customize culture media with reduced components or changes in the concentration of existing components.
