PB180424
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Product Introduction
Background
Amino Acids are the basic unit of protein composition and also an important component of life. According to the cell's own ability to synthesize, amino acids can be divided into three categories: essential amino acids, semi-essential amino acids, and non-essential amino acids.
MEM non-essential amino acid solution (NEAA) contain L-alanine, L-glutamic acid, L-asparaginate, L-proline, L-aspartic acid, L-serine and glycine, can effectively improve the ratio of cells culture medium, reduce the side effect of non-essential amino acid production in cell culture, and promote cell proliferation and metabolism, which is one of the commonly used additives in cell culture.
Ingredient Statement
With
• Ultrapure water Dissolvent
Matters Need Attention
1. This product is only used for scientific research or further research, not for diagnosis and treatment.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
4. This product is 100 × concentrated solution. Please dilute it to to the desired concentration before use.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's MEM Non-Essential Amino Acid Solution (NEAA), 100 × complies with ISO 13485, ISO 9001.
Specifications
| Concentration | 100× |
| Volume | 100mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 0.6-1.7 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 12 months |
| Storage Condition | This product can be stored at 2-8℃ for 12 months. |
| Shipping Condition | Ice bag |
Publications
Journal: PHARMACOLOGICAL RESEARCH (2026) IF: 10.5
DOI: 10.1016/j.phrs.2026.108154
Product Cited: MEM Non-Essential Amino Acid Solution (NEAA), 100 ×, DMEM (Glucose free) Medium
Journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2026) IF: 8.5
DOI: 10.1016/j.ijbiomac.2026.150861
Product Cited: Fetal Bovine Serum, DMEM/F12 Medium, Donor Equine Serum, McCoy's 5A Medium, RPMI-1640 Medium, EGF/Urogastrone/URG, Human, Recombinant, MCF 10A Cells Line, 10 mg/mL Recombinant Human Insulin Solution, MDA-MB-231 Cells Line, MEM Non-Essential Amino Acid Solution (NEAA), 100 ×, MEM Medium, MCF7 Cells Line, MDA-MB-468 Cells Line, DMEM (High glucose) Medium, 293T Cells Line
Journal: APOPTOSIS (2026) IF: 8.1
DOI: 10.1007/s10495-025-02225-7
Product Cited: Leibovitz's L-15 Medium, MDA-MB-468 Cells Line, Fetal Bovine Serum, 293T Cells Line, MEM Non-Essential Amino Acid Solution (NEAA), 100 ×, DMEM/F12 Medium, MCF7 Cells Line, MDA-MB-231 Cells Line, MEM Medium, Penicillin-Streptomycin Solution, 100 ×, MCF 10A Cells Line, Donor Equine Serum, DMEM (High glucose) Medium
Journal: Cancer Cell International (2026) IF: 6
DOI: 10.1186/s12935-025-04149-3
Product Cited: MEM Non-Essential Amino Acid Solution (NEAA), 100 ×, MCF7 Cells Line, MDA-MB-453 Cells Line, BT-474 Cells Line, MDA-MB-231 Cells Line, BT-549 Cells Line, SK-BR-3 Cells Line, 293T Cells Line, MDA-MB-468 Cells Line, MEM Medium, ZR-75-1 Cells Line
Journal: Frontiers in Immunology (2026) IF: 5.9
DOI: 10.3389/fimmu.2026.1735908
Product Cited: 10 mg/mL Recombinant Human Insulin Solution, MEM Non-Essential Amino Acid Solution (NEAA), 100 ×
FAQs
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Q:1 The MEM Non-Essential Amino Acids (NEAA) solution is based on the MEM formulation. Can it only be added to MEM medium, or is it also compatible with other culture media such as DMEM?
AnswerThe MEM NEAA solution can be added to media other than MEM. Since DMEM is a modified version of MEM and contains approximately twice the amino acid concentration—including non-essential amino acids—additional supplementation with NEAA is generally not necessary. However, under specific experimental conditions, such as when cells have a higher demand for amino acids or when culturing certain sensitive cell lines, it may be appropriate to supplement DMEM with MEM-derived NEAA.
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Q:2 What is the solvent used for MEM Non-Essential Amino Acids Solution (100X)?
AnswerThe solvent is purified water.
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Q:3 What is MEM Non-Essential Amino Acids?
AnswerMEM Non-Essential Amino Acids are used as a growth supplement for cell culture medium, to increase cell growth and viability. MEM Non-Essential Amino Acids contains the same non-essential amino acids found in the standard Minimum Essential Medium (MEM) at a strength of 100X.
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Q:4 What is the serial number and host for Recombinant Human Insulin Solution (10mg/ml)?
AnswerThe sequence number of this insulin is P01308, it is full-length, and the expression host is yeast.
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Q:5 If I use Pricella's B-27 (50x) to prepare Neurobasal + 2% B-27(50x), how do I prepare it? Is it 2% based on 50x or 2% based on 1x?
Answer2% based on 50×. For example, prepare 100 mL medium means adding 2 mL B-27(50×), the concentration is 2%.
