Phosphate Buffer (PBS, 1 ×)

Name: Phosphate Buffer (PBS, 1 ×)
Brand: Procell
SKU: PB180327
Price: 8.0 USD
Availability: InStock

Cat. No.: PB180327

Publications(491)

Manual

PB180327

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Product Introduction Documents Publications FAQs

Product Introduction

Background

Balanced Salt Solution (Physiological Solution) have the properties of buffer solution (regulate pH), normal saline (maintain osmotic pressure) and culture medium (provide nutrition). It can meet the basic needs of survival and metabolism of tissues, organs or cells in vitro. A small amount of phenolic red was added to some equilibrium salt solutions to indicate the pH change of the solution.
Phosphate Buffered Saline (PBS) is one of the most widely used equilibrium salt solutions in biochemistry. The main components are Na₂HPO₄、KH₂PO₄、NaCl and KCl. PBS buffers are often used to rinse tissue or cells, to transport cells or tissues, to prepare other reagents, to dilute the cells when counting the cells.

Ingredient Statement

Without

• NaHCO₃

• Phenol red

• Ca²⁺

• Mg²⁺

Matters Need Attention

1. This product is only used for scientific research or further research, not for diagnosis and treatment.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.

System Certification

GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's Phosphate Buffer (PBS, 1 ×) also meets the medical device registration certificate requirements and complies with ISO 13485, ISO 9001.

Specifications

Concentration	1×
Volume	500mL 1000mL
Form	Liquid
Bacteria Detection	Negative
Fungi Detection	Negative
Mycoplasma Detection	Negative
Endotoxin Content	< 1 EU/mL
pH	7.2-7.4
Animal Origin Ingredient	Without
Green Features	Sustainable packaging
Valid Period	36 months
Storage Condition	This product can be stored at 2-30℃ for 36 months.
Shipping Condition	Room Temperature

Documents

Manual

MSDS

Formulation

Publications

Expression of Neuron-Specific Enolase in Multiple Myeloma and Implications for Clinical Diagnosis and Treatment

Journal: PLoS One (2014) IF: 3.534

DOI: 10.1371/journal.pone.0094304

Product Cited: Phosphate Buffer (PBS, 1 ×)

FAQs

Q:1 What is the difference between PBS and DPBS?
Answer
PBS and DPBS both primarily consist of NaCl, KCl, KH₂PO₄, and Na₂HPO₄. Both solutions are available in formulations with or without calcium and magnesium ions; solutions containing calcium and magnesium ions should not be used for cell digestion. DPBS (PB180329) has a composition similar to PBS (PB180327). PB180330, PB180331, and PB180332 have reduced phosphate content compared with standard PBS formulations. DPBS is available in formulations with or without phenol red. DPBS (PB180332) contains glucose; refer to the formulation specifications for proper differentiation. In general, when no specific requirements are present and the solution is used solely for cell washing, both DPBS (PB180329) and PBS (PB180327) are suitable.
Q:2 Are PB solution and PBS solution the same and do they have identical applications?
Answer
No, PB (phosphate buffer) and PBS (phosphate-buffered saline) are not the same. PB solution consists primarily of phosphate buffering components only. In contrast, PBS contains phosphate buffer plus additional physiological salts, such as sodium chloride and potassium chloride, which make it more similar to physiological ionic conditions. For applications such as reagent preparation or tissue washing, PBS is generally preferred due to its balanced osmolarity and ionic composition, which better supports biological samples.
Q:3 Is the 1× phosphate-buffered saline (PBS) sterile and nuclease-free?
Answer
It is sterile but not nuclease-free, as it has not undergone nuclease removal treatment.
Q:4 My PBS was accidentally frozen. Can I still use it?
Answer
This product should be stored at 2-8℃ and should not be frozen. The biggest issue with accidentally freezing this product is its solubility. If this product was accidentally frozen, we recommend placing it in a 2-8℃ environment and allowing it to slowly thaw overnight. If the product is fully thawed and shows no signs of precipitation, then it should still be usable, but we cannot guarantee effectiveness.
Q:5 Are your PBS products tested for endotoxin levels?
Answer
Our PBS products are tested for endotoxin and the endotoxin levels are shown on the Certificate of Analysis (CoA) for the particular lot number of the product.