RPMI-1640

Cat. No.: PM150110 Publications(1005) Manual
RPMI-1640 - 1
  • RPMI-1640RPMI-1640 - 1

PM150110

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500mL

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Product Introduction

Background

RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media.
RPMI-1640 medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used serum or serum-free additives.

Ingredient Statement
With

• 2000 mg/L D-Glucose

• 2 mM L-Glutamine

• 2000 mg/L NaHCO₃

• 5 mg/L Phenol red

Without

• HEPES

Matters Need Attention

1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.

System Certification

GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's RPMI-1640 also meets the medical device registration certificate requirements and complies with ISO 13485, ISO 9001.

Specifications
Concentration
Volume 500mL
Form Liquid
Bacteria Detection Negative
Fungi Detection Negative
Mycoplasma Detection Negative
Endotoxin Content < 3 EU/mL
pH 7.2-7.4
Animal Origin Ingredient Without
Green Features Sustainable packaging
Valid Period 24 months
Storage Condition This product can be stored at 2-8℃ for 24 months with shading light.
Shipping Condition Room Temperature

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Documents

Publications

Intermediate filament IFFO1 negatively regulates the migration of lung cancer cells by inhibiting the IQGAP3-Cdc42 interaction

Journal: Cell Death & Disease (2025) IF: 9.6

DOI: 10.1038/s41419-025-07846-z

Product Cited: NCI-H358 Cells Line, NCI-H1299 Cells Line, McCoy's 5A Medium, Calu-1 Cells Line, RPMI-1640 Medium, NCI-H292 Cells Line, DMEM (High glucose) Medium

Micropeptide hSPAR regulates glutamine levels and suppresses mammary tumor growth via a TRIM21-P27KIP1-mTOR axis

Journal: EMBO JOURNAL (2025) IF: 9.4

DOI: 10.1038/s44318-024-00359-z

Product Cited: MCF 10A Cells Line, MDA-MB-468-06 Cells Line, MCF 10A Cell Complete Medium, MDA-MB-453 Cells Line, 293T [HEK-293T] Cells Line, MCF7 [MCF-7] Cell Complete Medium, MDA-MB-231 Cells Line, RPMI-1640 Medium, MCF7 [MCF-7] Cells Line

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