SILAC RPMI-1640 Medium, without glucose, phenol red
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PM150144
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Product Introduction
Background
RPMI-1640 is an improved McCoy's 5A medium, which uses bicarbonate buffer system. RPMI-1640 medium was originally designed for lymphocyte culture. Nowadays, it has been widely used in the culture of normal cells and cancer cells, especially suspension cells, which is one of the most widely used media.
Stable isotope labeling with amino acids (SILAC) in cell culture is a technique for quantitative analysis of protein expression using stable isotope-labeled amino acid binding mass spectrometry during cell culture. SILAC RPMI-1640 Flex Media is improved on the basis of RPMI-1640 medium. The medium does not contain L-glutamine, L-arginine, L-lysine, glucose and phenol red. It is often used in the study of stable isotope-labeled proteins of arginine and lysine.
Phenol red is used as an indicator of pH in the medium to continuously monitor the pH of the medium. The medium is yellow at low pH, and is purple at high pH. When the pH is 7.2-7.4, it is red, which is most suitable for cell culture. But phenol red also has some disadvantages, studies have shown that phenol red can simulate the role of steroid hormones (especially estrogen), so it is best to use the medium without phenol red to culture estrogen-sensitive cells (such as breast tissue). Second, phenol red will interfere with flow cytometry analysis. In addition, the presence of phenol red in some serum-free medium formulations can interfere with the sodium-potassium balance.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 2000 mg/L NaHCO₃
Without
• D-Glucose
• HEPES
• L-Glutamine
• Phenol red
• L-Arginine
• L-Lysine
Matters Need Attention
1. This product is only used for scientific research or further research, not for diagnosis and treatment.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's SILAC RPMI-1640 Medium, without glucose, phenol red complies with ISO 13485, ISO 9001.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Related Products
Documents
Publications
Journal: PHYTOMEDICINE (2026) IF: 8.3
DOI: 10.1016/j.phymed.2026.158145
Product Cited: SILAC RPMI-1640 Medium, without glucose, phenol red, RPMI-1640 Medium
FAQs
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Q:1 Can basic culture medium be used if it is accidentally frozen at -20°C?
AnswerIf the cell culture medium is accidentally frozen, allow it to thaw completely at room temperature and gently mix before use. After thawing, check whether any precipitation has formed. If no precipitate is observed, the medium can be used normally. If precipitation is present, it indicates that certain components have precipitated out of solution; in this case, to avoid affecting cell growth, the medium is not recommended for further use.
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Q:2 If the basic culture medium is frozen at -20℃, will it affect its use?
AnswerIf the cell culture medium is accidentally frozen, observe whether there is precipitation after the medium is naturally thawed. If no precipitation is produced, the medium can be used normally. If precipitation occurs, it is recommended to discard it.
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Q:3 How long can I keep my culture media after supplementing with serum?
AnswerGenerally speaking, media can be used for up to 3-6 months after supplementation with serum， at 2 to 8℃ low temperature environment preservation.
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Q:4 My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?
AnswerWe routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
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Q:5 How light sensitive is media? Should I also be protecting it from LED light?
AnswerWhile we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.When placed in a light-proof 4°C refrigerator with the door closed, the culture medium will be kept away from light.
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Q:6 I see a precipitate in my medium, and a change in the pH. What should I do?
AnswerThis is most likely a sign of bacterial or fungal contamination. We would recommend discarding the media, and trying to decontaminate the culture.
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Q:7 Where can I find the osmolality for Medium?
AnswerThe osmolality is listed in the COA for the particular lot number of the medium.
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Q:8 Do you provide customized culture media services?
AnswerYes. We can customize culture media with reduced components or changes in the concentration of existing components.
