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CL-0247
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Product Introduction
| Cell Name | ZR-75-1 |
| Synonyms | ZR751;ZR75-1;ZR75_1 |
| Cellosaurus Accession | CVCL_0588 |
| Organism | Human |
| Growth Properties | Adherent |
| Morphology | Epithelial-like |
| Disease | breast cancer cells |
| Tissue | Mammary gland/breast; derived from metastatic site: ascites |
| Age | 63Y |
| Sex | Female |
| Instructions | 1. Check all containers for leakage or breakage. 2. Remove the frozen cells from the dry ice packaging and immediately transfer them to liquid nitrogen (liquid or vapor phase) for long-term cryopreservation. 3. This cell line exhibits weak adherence, so all manipulations must be carried out gently. Pre-warm the culture medium before medium exchange. |
| Complete Medium | RPMI-1640 [PM150110]+10% Nutrient+1% Supplement |
| Incubation Atmosphere | Air, 95%; CO₂, 5% |
| Temperature | 37℃ |
| Subcultivation Ratio | 1:2-1:3 |
| Medium Renewal | 2 to 3 times per week |
| Dissociation Duration | 4-5 min |
| Subculturing Procedure | 1. Remove the culture medium from the T25 cell culture flask. 2. Add approximately 2 mL of PBS. Gently tilt the flask side to side until the PBS covers the entire bottom, then aspirate and discard the PBS. 3. Add 1 mL of 0.25% trypsin solution (containing EDTA). Gently tilt the flask side to side until the trypsin solution covers the entire bottom of the flask. 4. Incubate the cells at 37°C. Observe the cells under an inverted microscope and terminate digestion once the cells round up and detach. To avoid clumping, do not agitate the cells by tapping or shaking the flask during detachment. 5. Add 3 mL of complete culture medium to terminate digestion and disperse into a single cell suspension. 6. Collect the cell suspension and centrifuge at 1200 rpm (approximately 250 ×g) for 3 minutes. Carefully aspirate and discard the supernatant. 7. Add fresh complete culture medium, pipette gently several times to resuspend the cells, and seed them at the appropriate ratio into a new culture flask. Loosen the cap or use a vented cap for incubation. |
| Freeze Medium | General Freezing Medium [PB180436] |
| Storage Conditions | For long-term cryopreservation, cryovials should be stored in liquid nitrogen at −150°C to −196°C. Storage at −80°C is restricted to short-term interim use only. |
| Background | The ZR-75–1 cell line produces high levels of MUC1 mucin mRNA and low levels of MUC2 mRNA, but does not express the MUC3 gene. ZR-75–1 cells are positive for estrogen receptor (ER) expression. |
| Tumorigenic | Yes, forms tumors in nude mice. |
| Receptor Expression | estrogen receptor, expressed |
| Gene Expression | mucin (apomucin, MUC-1, MUC-2) |
| Biosafety Level | BSL-1 |
Documents
MSDSPublications
Journal: MOLECULAR CARCINOGENESIS (2024) IF: 4.6
DOI: 10.1002/mc.23724
Product Cited: MDA-MB-468-06 Cells Line, BT-549 Cells Line, T-47D Cells Line, MDA-MB-231 Cells Line, ZR-75-1 Cells Line, MCF7 [MCF-7] Cells Line, MCF 10A Cells Line
Journal: Scientific Reports (2024) IF: 3.8
DOI: 10.1038/s41598-024-79149-w
Product Cited: HCC1806 Cells Line, RPMI-1640 Medium, Penicillin-Streptomycin Solution, 100 ×, ZR-75-1 Cells Line, MCF7 [MCF-7] Cells Line, A549 [A-549] Cells Line, Fetal Bovine Serum, MEM Complete Medium (10), SUM159PT Cells Line, MDA-MB-231 Cells Line, JIMT-1 Cells Line, T-47D Cells Line, DMEM (High glucose) Medium
Journal: BMC CANCER (2024) IF: 3.4
DOI: 10.1186/s12885-024-13290-7
Product Cited: RAW 264.7 Cells Line, ZR-75-1 Cells Line, MDA-MB-231 Cells Line
Journal: Molecular Cancer (2023) IF: 37.3
DOI: 10.1186/s12943-023-01806-x
Product Cited: MDA-MB-231 Cells Line, MDA-MB-468 Cells Line, 293T [HEK-293T] Cells Line, Hs 578T Cells Line, MCF7 [MCF-7] Cells Line, T-47D Cells Line, ZR-75-1 Cells Line, MCF 10A Cells Line
Journal: Experimental and Therapeutic Medicine (2023) IF: 2.7
Product Cited: MCF 10A Cells Line, MCF7 [MCF-7] Cells Line, MDA-MB-231 Cells Line, ZR-75-1 Cells Line, HCC1937 Cells Line, 293 [HEK-293] Cells Line
