DMEM/F12

Cat. No.: PM150312 Publications(350) Manual
DMEM/F12 - 1
  • DMEM/F12DMEM/F12 - 1

PM150312

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Product Introduction

Background

The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.

Ingredient Statement
With

• 3151 mg/L D-Glucose

• 2.5 mM L-Glutamine

• 2438 mg/L NaHCO₃

• 8.1 mg/L Phenol red

• 0.5 mM Sodium pyruvate

Without

• HEPES

Matters Need Attention

1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.

System Certification

GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's DMEM/F12 also meets the medical device registration certificate requirements and complies with ISO 13485, ISO 9001.

Specifications
Concentration
Volume 500mL 1000mL
Form Liquid
Bacteria Detection Negative
Fungi Detection Negative
Mycoplasma Detection Negative
Endotoxin Content < 3 EU/mL
pH 7.2-7.4
Animal Origin Ingredient Without
Green Features Sustainable packaging
Valid Period 24 months
Storage Condition This product can be stored at 2-8℃ for 24 months with shading light.
Shipping Condition Room Temperature

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Publications

FGF2 Rescued Cisplatin-Injured Granulosa Cells through the NRF2-Autophagy Pathway

Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2023) IF: 5.6

DOI: 10.3390/ijms241814215

Product Cited: KGN Cells Line, DMEM/F12

DEPDC1B-mediated USP5 deubiquitination of β-catenin promotes breast cancer metastasis by activating the wnt/β-catenin pathway

Journal: AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY (2023) IF: 5.5

DOI: 10.1152/ajpcell.00249.2023

Product Cited: MDA-MB-231 Cells Line, MDA-MB-468 Cells Line, MDA-MB-157 Cells Line, BT-549 Cells Line, 293 [HEK-293] Cells Line, DMEM/F12, Hs 578T Cells Complete Medium

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