PM150312
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Product Introduction
Background
The DMEM/F12 medium is based on the DMEM medium, adding more nutritious elements from F12 medium, which contains a variety of trace elements, and is widely used in the culture of many mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium. It is also suitable for mammalian cell culture and clone density culture under low serum content.
This product contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
Ingredient Statement
With
• 3151 mg/L D-Glucose
• 2.5 mM L-Glutamine
• 2438 mg/L NaHCO₃
• 8.1 mg/L Phenol red
• 0.5 mM Sodium pyruvate
Without
• HEPES
Matters Need Attention
1. This product is for research use only.
2. This product is sterilized by 0.1 μm filtration.
3. It is necessary to pay attention to the aseptic operation and avoid the contamination.
System Certification
GMP Production and Quality System
The company complies with cGMP requirements, holds the NMPA medical device registration certificate, and is certified under ISO 13485 and ISO 9001 standards. Among its products, the company's DMEM/F12 also meets the medical device registration certificate requirements and complies with ISO 13485, ISO 9001.
Specifications
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.2-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 24 months |
| Storage Condition | This product can be stored at 2-8℃ for 24 months with shading light. |
| Shipping Condition | Room Temperature |
Publications
Journal: JOURNAL OF ETHNOPHARMACOLOGY (2025) IF: 5.4
DOI: 10.1016/j.jep.2025.120435
Product Cited: McCoy's 5A Medium, DMEM (Glucose free), without sodium pyruvate Medium, DMEM/F12 Medium, DMEM (High glucose) Medium, H22 Cells Line, 293T Cells Line
Journal: Communications Biology (2025) IF: 5.1
DOI: 10.1038/s42003-025-08421-0
Product Cited: DMEM/F12 Medium, Fetal Bovine Serum, DMEM (High glucose) Medium
Journal: Signal Transduction and Targeted Therapy (2025) IF: 40.8
DOI: 10.1038/s41392-025-02250-7
Product Cited: DMEM (High glucose) Medium, MEM, with NEAA Medium, Penicillin-Streptomycin Solution, 100 ×, McCoy's 5A Medium, DMEM/F12 Medium, 50 mg/mL G-418 Solution
Journal: JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY (2025) IF: 4.9
DOI: 10.1016/j.jddst.2025.107215
Product Cited: Penicillin-Streptomycin Solution, 100 ×, DMEM/F12 Medium, Phosphate Buffer (PBS, 1 ×), 0.25% Trypsin Solution, with EDTA, phenol red, dissolved in D-Hank's
Journal: Frontiers in Bioengineering and Biotechnology (2025) IF: 4.8
DOI: 10.3389/fbioe.2025.1611948
Product Cited: DMEM/F12 Medium
